Jack Grimmer and Port Vale went out of the FA Cup with Budweiser on Saturday after a 3-1 Fourth Round defeat at home to Brighton & Hove Albion.
In wet conditions, Rohan Ince's hooked finish put the Sky Bet Championship side ahead at their League One counterparts, but Chris Robertson headed in from six yards to level.
A mis-hit Solly March cross floated into the top corner to restore Brighton's lead before the break and Jonathan Obika rounded goalkeeper Chris Neal to seal the win with 12 minutes remaining.
Cauley Woodrow was a 70th-minute substitute as Southend United saw their cup dreams end with a 2-0 defeat at home to Hull City. Matty Fryatt scored Hull’s goals in the 63rd and 90th minutes.
In League One, Stephen Arthurworrey played the full 90 as Tranmere Rovers earned a first victory in six games with a 1-0 win over fellow strugglers Crewe Alexandra. On-loan Everton defender Matthew Pennington's header secured Rovers the three points as they climbed up to 17th.
In the same division on Friday night, Marcello Trotta was on target once again as Brentford moved back to the top of League One with a 2-1 win over Gillingham.
Jonathan Douglas broke the deadlock for the Bees in the 22nd minute when he glanced home Jake Bidwell's cross. And Trotta slotted home from the spot with 20 minutes remaining after Tony Craig had been fouled in the box by Adam Barrett.
Cody McDonald's strike set up a tense finale but Brentford held on to make it nine home wins in a row.
A division lower, Ryan Williams claimed an assist on an eventful day for Oxford United in their 1-0 win over Torquay United.
Williams ran through the centre of the Torquay defence before feeding Deane Smalley to score what proved to be the winner with a simple finish after just six minutes.
The result saw Oxford climb to sixth place in League Two but, shortly after the final whistle, manager Chris Wilder resigned from his position.
Marcus Bettinelli and Accrington Stanley saw their home game with Bury postponed due to a waterlogged pitch.
Finally, Bryan Ruiz made his home debut for PSV Eindhoven as they defeated AZ Alkmaar 1-0 to climb up to sixth in the Eredivisie. Jetro Willems scored the only goal of the game in the second minute, with Ruiz replaced in injury time.